Download e-book for iPad: Actions of Progesterone on the Brain by Thomas Rabe, Ludwig Kiesel, Benno Runnebaum (auth.), Dr.

By Thomas Rabe, Ludwig Kiesel, Benno Runnebaum (auth.), Dr. Detlev Ganten M.D., Ph.D., Dr. Donald Pfaff Ph.D. (eds.)

ISBN-10: 3642697283

ISBN-13: 9783642697289

ISBN-10: 3642697305

ISBN-13: 9783642697302

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5) and centrifuged at 800 g for 20 min. 25 M sucrose-TMKG buffer. 25 M sucrose-TMKG buffer and was used for the measurement of nuclear estrogen receptors. , Tokyo) to yield the high-speed supernatant (cytosol). The cytosol fraction was then treated with dextran-coated charcoal to remove free hormone. All subsequent procedures, unless otherwise noted, were performed at 4 DC. Sucrose Density Gradient Centrifugation. 1 Ci/mmol] in the absence or presence of a 100-fold excess of unlabeled steroid hormones dissolved in 5 Jll ethanol.

The cytosols were incubated at 0 °C for 4 h in the presence of varying concentrations of [3H] RS020 or [3H] RS020 with a 100-fold excess of RS020. The amounts of specifically bound [3H]S020, were measured by dextran-coated charcoal assay, and plotted by the method of Scatchard (1949). (1) PO-Ant. 8 fmol/ mg p; (2) Post.

1 Materials and Methods Monkey Treatment and Brain Dissection. 0 kg, were used. The monkeys were fed once a day with monkey chow, and twice a week with vegetables and fruit. ), and an antibiotic (Keflex, Shionogi, 150 mg/ day) was given once a day for 3 consecutive days. c. g 17f3-estradiol benzoate (Pellanin, Mochida Seiyaku, Tokyo) in sesame oil; for 4-14 consecutive days. ) 24 h after the last injection of estradiol benzoate: the brain and hypophysis were removed, rinsed in icecold physiological saline and placed on an ice-cold glass plate.

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Actions of Progesterone on the Brain by Thomas Rabe, Ludwig Kiesel, Benno Runnebaum (auth.), Dr. Detlev Ganten M.D., Ph.D., Dr. Donald Pfaff Ph.D. (eds.)

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