By Sameh Magdeldin
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Extra info for Affinity chromatography
At the beginning of the topic describing immobilization techniques and their advantages we should explain the meaning of the word "immobilization" in this text. A molecule or a cell is referred to be immobilized, if its mobility in the reaction space is artificially restricted. Many various immobilization protocols were evolved as we will show in the next chapter part. ) and various cells and cellular organelles may be immobilized. In some cases also systems containing more than one immobilized enzyme were prepared.
Anal Biochem 404: 106-108 Janson, J-C. (1984) Large-scale affinity purification — state of the art and future prospects. Trends in Biotechnology 2: 31-38 Jin W, Brennan JD (2002) Properties and applications of proteins encapsulated within sol–gel derived materials. Analytica Chimica Acta 461: 1-36 Katoh S, Imada M, Takeda N, Katsuda T, Miyahara H, Inoue M, Nakamura S (2007) Optimization of silica-based media for antibody purification by protein A affinity chromatography. J Chromatogr A 1161: 36-40 Kim, HS, Hage, DS, Immobilization methods for affinity chromatography, In: Handbook of Affinity Chromatography, Hage, DS, pp 35-78, Taylor & Francis, Boca Raton, FL.
J Proteome Res 9: 4982-4991 Tyutyulkova S, Paul S (1995) Purification of antibody light chains by metal affinity and protein L chromatography. Methods Mol Biol 51: 395-401 Udomsinprasert R, Pongjaroenkit S, Wongsantichon J, Oakley AJ, Prapanthadara LA, Wilce MC, Ketterman AJ (2005) Identification, characterization and structure of a new Delta class glutathione transferase isoenzyme. Biochem J 388: 763-771 Wang G, De J, Schoeniger JS, Roe DC, Carbonell RG (2004) A hexamer peptide ligand that binds selectively to staphylococcal enterotoxin B: isolation from a solid phase combinatorial library.
Affinity chromatography by Sameh Magdeldin